Product Name: |
HRV3c protease |
|
Cat No.: |
IT-000-HRV3c |
Source: |
Recombinant protein from E.coli. |
Formulation: |
50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.04% Tween20 and 50% glycerol. |
Protein: |
N-terminal 6xHis tagged HRV 3C Protease (Human Rhinovirus 3C Protease, PreScission Site)(GenBank# P03303). |
Applications: |
The enzyme recognizes the cleavage site: Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro (LEVLFQ↓GP). The enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37℃. |
Activity: |
≥ 1 U/ l ml; No detectable RNAase, DNAase or other protease activity. |
Definition of Activity Unit: |
One unit of HRV3c Protease is defined as the amount of enzyme needed to cleave >95% of 100µg of substrate protein at 4°C in 16 hours. |
Storage: |
Kept at -20oC. Non-hazardous. No MSDS required. |